Topo I (Y723F) (mt Y723F) human

半岛bd体育手机客户端 说明

生化/生理作用

Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Tyrosine 723 was identified as an active site for the DNA binding activity of DNA topoisomerase I. The covalent intermediate of topo I and DNA complex includes nucleophilic attack by the O4-oxygen of tyrosine 723 on a phosphester linkage in the DNA. Mutation from tyrosine to phenylalanine at position 723 preferentially binds the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNAs. But mutation at tyr⁷²³neither affects its kinase activity that phosphorylates splicing factors of SR protein family nor its transcription activity of class II genesin vitro.

外形

Clear and colorless frozen liquid solution

制备说明

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

基本信息

NACRES

NA.26

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in insect cells
测定	≥80% (SDS-PAGE)
形式	frozen liquid
分子量	~92.4 kDa
包装	pkg of 5 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	1000 μg/mL
颜色	clear colorless
NCBI登记号	NM_003286
UniProt登记号	P11387
运输	dry ice
储存温度	?70℃
Gene Information	human ... TOP1(7150)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable