半岛bd体育手机客户端 说明
一般描述
TEV protease is a highly sequence specific serine protease from Tobacco Etch Virus. Due to its high specificity, TEV protease is popular for cleavage of recombinant fusion proteins. The optimal sequence for TEV protease cleavage is ENLYFQ\S is, however, TEV protease is active on a range of substrates with a consensus sequence of EXLYFΦQ\? where X is any residue, Φ is any large or medium hydrophobic residue and ? is any small hydrophobic or polar residue (i.e. glycine, serine, alanine, valine, cysteine). Fusion tag removal in-vitro is the most popular usage of TEV protease.
This biotin-tagged TEV protease is expressed in E.coli. Our Biotin-tagged TEV protease does not carry any purification tag other than biotin and is designed to be used for on-column cleavage of fusion proteins containing a TEV protease recognition sequence. This method specifically cleaves the protein of interest from a column-bound fusion protein, leaving the purification domain or tag bound to the affinity column (e.g. Ni-NTA column) and eluting only the protein of interest.
This method is advantageous to post-elution cleavage for several reasons:
单位定义
One unit of TEV protease cleaves >85% of 3 μg of control substrate in one hour at pH 8.0 at 30 ℃
半岛bd体育手机客户端 性质
运输 | wet ice |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich