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位置: 首页> 品牌> Sigma-Aldrich> PSF-TEFI-TPI1-NEO/G418-G418 YEAST SELECTION PLASMID

PSF-TEFI-TPI1-NEO/G418-G418 YEAST SELECTION PLASMID

品牌
Sigma-Aldrich
货号
OGS542
规格纯度
plasmid vector for molecular cloning
价格
4853.18 *本价格含增值税费
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半岛bd体育手机客户端 名称:
PSF-TEFI-TPI1-NEO/G418-G418 YEAST SELECTION PLASMID
expression vector
molecular cloning vector
vector
snapfast vector
plasmid vector
cloning vector
plasmid
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

This is a yeast expression plasmid containing the strong TEF1 constitutive promoter that allows for the expression of transgenes in Saccharomyces cerevisiae. The vector also contains a resistance gene for G418 and allows plasmids to be selected and grown in yeast cells using this antibiotic.
Promoter Expression Level:This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the antibiotic resistance gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).

应用

Cloning in a gene:This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes:There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

基本信息

NACRES NA.85

半岛bd体育手机客户端 性质

形式 buffered aqueous solution
分子量 size 7252 bp
复制起点 2Micron
pUC (500 copies)
肽切割 no cleavage
启动子 Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
菌种筛选 kanamycin
报告基因 none
运输 ambient
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
闪点(F) Not applicable
闪点(C) Not applicable

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