半岛bd体育手机客户端 说明
一般描述
Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80℃. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
外形
1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human |
| 重组 | expressed inE. coli |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | liquid |
| 分子量 | 39.4 kDa (18-333 aa + N-terminal poly-his tag.) |
| 包装 | pkg of 5 μg |
| 浓度 | 1 mg/mL |
| UniProt登记号 | P07711 |
| 运输 | wet ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... CTSL1(1514) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
