PCNA human

半岛bd体育手机客户端 说明

一般描述

PCNA (proliferating cell nuclear antigen) gene is mapped to human chromosome 20p12.3 and encodes a scaffolding protein. PCNA is a homotrimeric protein known to localize in the nucleus. The PCNA ring has a central cavity that is large enough to allow the dsDNA to slide freely along it.

生化/生理作用

Proliferating cell nuclear antigen (PCNA) has its main function in DNA replication and repair mechanism. Itsignificantly maintains the longevity of mature neutrophil granulocytes. Upregulation of the PCNA gene is observed in cervical squamous cell carcinoma. PCNA supports cell survival by elevating metabolism flux. This gene expression is known to offer resistance to chemotherapy. Expression of PCNA or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Recombinant PCNA was expressed with a 6x His tag in sf9 insect cells and purified by proprietary chromatographic techniques.

外形

Sterile filtered; 5 μg/5 μl in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA, and 20% Glycerol.

重悬

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in baculovirus infectedSf9 cells
测定	≥90% (HPLC) ≥90% (SDS-PAGE)
形式	liquid
分子量	~29.8 kDa
包装	pkg of 5 μg
杂质	endotoxin, tested
NCBI登记号	P12004
运输	dry ice
储存温度	?70℃
Gene Information	human ... PCNA(5111)