半岛bd体育手机客户端 说明
一般描述
Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 variant of MSP1 differs from MSP1 in the following facets:
应用
纳米盘可溶性脂质双层系统已被证明是一种广泛适用的手段,用于使膜蛋白溶在水溶液中的类似天然的双层膜环境中,维持其单分散和活性。纳米盘的关键组分是包绕着两亲性螺旋蛋白质带(膜支架蛋白)。
纳米盘系统已被用于包裹多种蛋白质,包括 GPCR、P450、细菌视紫红质、凝血因子、霍乱毒素、TAR 受体和芳香酶。
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
生化/生理作用
生成直径约 12.9nm 的纳米盘
法律信息
纳米盘技术,以及它的许多用途,均由伊利诺伊大学所有的下列专利所保护。
基本信息
NACRES | NA.26 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
重组 | expressed inE. coli |
测定 | ≥90% (SDS-GE) |
形式 | buffered aqueous solution |
运输 | ambient |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich