您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4

Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4

品牌
Sigma-Aldrich
货号
05-1242
规格纯度
clone 6F12-H4, from mouse
价格
7365.52 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4
H3K9me3, Histone H3 (tri methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely via acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α/β. The enzymes responsible for H3K9me3 formation are SUV39H1 and SUV39H2.

特异性

Based on sequence homology, broad species cross-reactivity is expected with all mammals, Drosophila, Xenopus, and Arabidopsis.

应用

Use Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4 (mouse monoclonal antibody) validated in ChIP, PIA, IF, DB, ChIP-seq, WB to detect trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9).
Chromatin Immunoprecipitation (ChIP):
Representative data from a previous lot. Sonicated 3T3 L1 chromatin was subjected to chromatin immunoprecipitation using anti- trimethyl-histone H3 (Lys9) and the Magna ChIP G (Cat. #17-611) Kit. Successful immunoprecipitation of trimethylhistone H3 (Lys9) associated DNA fragments was verified by qPCR using primers flanking the p16 promoter.
Peptide Inhibition Analysis:
Peptide blocking assay demonstrates distinct preference of the antibody for the trimethyl form vs. the dimethyl form.
Chromatin Immunoprecipitation (ChIP):
ChIP analysis of known chromosomal Suv39h targets (H3K9me3 in major satellites, mouseES cells).
Dot Blot Analysis:
Dot-blot analysis demonstrating specificity of anti-H3K9me3, clone 6F12-H4 for trimethyl Lys9 of histone H3.

质量

Routinely evaluated by Western Blot on HeLa acid extracts.
Western Blot Analysis: A 0.5 – 5 μg dilution of this lot detected trimethyl histone H3 (Lys9) in HeLa acid extracts.

目标描述

~17 kDa

外形

Purified
Format: Purified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 6F12-H4, monoclonal
species reactivity mouse, human
technique(s) ChIP: suitable (ChIP-seq)
dot blot: suitable
immunofluorescence: suitable
inhibition assay: suitable (peptide)
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NM_003493
UniProt登记号 Q16695
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map