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位置: 首页> 品牌> Sigma-Aldrich> Anti-Histone H1 Antibody, clone AE-4

Anti-Histone H1 Antibody, clone AE-4

品牌
Sigma-Aldrich
货号
05-457
规格纯度
clone AE-4, Upstate?, from mouse
价格
7547.03 *本价格含增值税费
促销
服务
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  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Anti-Histone H1 Antibody, clone AE-4
H1 histone family, member 4, Histone H1b, histone 1, H1e, histone cluster 1, H1e
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

特异性

Predicted to stain nuclei from other animal and plant species.
This antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B > H4 > H2A, as determined with recombinant protein.

免疫原

Nuclei from myeloid leukemia biopsy cells. Clone AE-4.

应用

Use Anti-Histone H1 Antibody, clone AE-4 (mouse monoclonal antibody) validated in ELISA, ICC, WB to detect Histone H1 also known as H1 histone family member 4, Histone H1b, histone 1 H1e, histone cluster 1 H1e.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry:
5-20 ?g/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.
ELISA:
Recommended.

质量

Routinely evaluated by immunoblot on lysates from an acid extract of HeLa cells.
Western Blot:
1-4 ?g/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.

目标描述

32-33 kDa (Histone H1); Doublet (hyperphosphorylated Histone H1); 17 kDa (other histone isoforms).

外形

Purified mouse monoclonal IgG2a in buffer containing PBS, pH 7.2, with 0.1% sodium azide. Frozen solution.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Nuclear extracts from NIH3T3 or HeLa cells.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 AE-4, monoclonal
species reactivity plant, bovine, human
manufacturer/tradename Upstate?
technique(s) ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型 IgG2a
NCBI登记号 NM_005322
UniProt登记号 P10412
运输 dry ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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