半岛bd体育手机客户端 说明
一般描述
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
特异性
Predicted to stain nuclei from other animal and plant species.
This antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B > H4 > H2A, as determined with recombinant protein.
免疫原
Nuclei from myeloid leukemia biopsy cells. Clone AE-4.
应用
Use Anti-Histone H1 Antibody, clone AE-4 (mouse monoclonal antibody) validated in ELISA, ICC, WB to detect Histone H1 also known as H1 histone family member 4, Histone H1b, histone 1 H1e, histone cluster 1 H1e.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry:
5-20 ?g/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.
ELISA:
Recommended.
质量
Routinely evaluated by immunoblot on lysates from an acid extract of HeLa cells.
Western Blot:
1-4 ?g/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.
目标描述
32-33 kDa (Histone H1); Doublet (hyperphosphorylated Histone H1); 17 kDa (other histone isoforms).
外形
Purified mouse monoclonal IgG2a in buffer containing PBS, pH 7.2, with 0.1% sodium azide. Frozen solution.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Nuclear extracts from NIH3T3 or HeLa cells.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | AE-4, monoclonal |
species reactivity | plant, bovine, human |
manufacturer/tradename | Upstate? |
technique(s) | ELISA: suitable immunocytochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2a |
NCBI登记号 | NM_005322 |
UniProt登记号 | P10412 |
运输 | dry ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich