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位置: 首页> 品牌> Sigma-Aldrich> 抗 谷胱甘肽-S-转移酶

Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit

抗 谷胱甘肽-S-转移酶

品牌
Sigma-Aldrich
货号
A7340
规格纯度
IgG fraction of antiserum, buffered aqueous solution
价格
10576.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit
Anti-Glutathione-S-Transferase
Anti-GST
抗 谷胱甘肽-S-转移酶
抗 GST
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

该抗体对日本血吸虫谷胱甘肽-S-转移酶的天然型和变性-还原型均具有特异性。抗 GST 可用于各种免疫分析,以鉴定 GST 融合蛋白的表达。
Glutathione-S-Transferase (GST) belongs to a family of Phase II detoxification enzymes which are involved in catalyzing the conjugation of glutathione (GSH) with many exogenous and endogenous electrophilic substances. GST is of two kinds- membrane-bound microsomal and cytosolic. The cytosolic GSTs are made of six classes: α, μ, ω, π, θ, and ζ.

特异性

Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.

免疫原

日本血吸虫的重组 GST 在大肠杆菌中表达

应用

Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used in

  • immunoblotting
  • affinity pull-down assay
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

生化/生理作用

Glutathione-S-Transferase (GST) are involved in protecting cellular macromolecules from the reactive electrophiles. The μ and π transferases regulate the mitogen-activated protein (MAP) kinase pathway. GSTs help in building up cellular resistance against microbial antibiotic, herbicides, insecticides, chemotherapy agents and drugs.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

制备说明

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al.,Scand. J. Immunol.,8, Suppl. 7, 7 (1978).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.46

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 peroxidase conjugate
抗体形式 IgG fraction of antiserum
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen 27.5 kDa
technique(s) direct ELISA: 1:10,000
western blot: 1:10,000 using lysates ofEscherichia coliinduced to express recombinant GST
运输 dry ice
储存温度 ?20℃

安全信息

象形图 GHS08
警示用语: Danger
危险声明 H317 - H334
预防措施声明 P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
危险分类 Resp. Sens. 1 - Skin Sens. 1
储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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