Anti-Giα2 Antibody, clone L5

半岛bd体育手机客户端 说明

特异性

Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
This antibody recognizes Giα-2.

免疫原

Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).

应用

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.
Research Category
Signaling
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer
Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes

质量

Routinely evaluated by Western Blot on Huvec lysates.
Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

目标描述

Labels a single protein band of 39-42 kDa in bovine and rat membranes.

联系

Replaces: MAB3077

外形

Purified
Format: Purified
Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.

储存及稳定性

Maintain at 2-8°C for 1 year from date of receipt.

分析说明

Control
Huvec cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	L5, monoclonal
species reactivity	mouse (69%), human (62%), guinea pig (43%), bovine (100%), rat (69%)
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG2b
NCBI登记号	NM_002070.2
UniProt登记号	P04899
运输	wet ice
Gene Information	human ... GNAI2(2771)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable