Anti-erbB4 Antibody, clone HFR1/2G4

半岛bd体育手机客户端 说明

一般描述

v-ErbB was identified as the viral counterpart of the EGF Receptor, and its relatives have adopted this nomenclature (or the HER nomenclature for human proteins). These proteins are activated by Heregulin, Neuregulin and Amphiregulin. ErbB-2/HER-2, also known as neu, is amplified in large numbers of highly aggressive breast cancers, but all the family members are capable of heterodimerization, and together activate downstream signaling pathways. ErbB-3 has a natural "substitution" at the codon which is an invariant Lys residue at the ATP-binding site of all protein kinases. It is therefore inactive as a kinase, and can only become phosphorylated by heterodimerization with another erbB-family member. All members of this family, when Tyr-phosphorylated, can nucleate the formation of signaling complexes through adapter proteins.

特异性

Reactive with human c-erB-4/Her 4 cytoplasmic domain of the protein (residues 1250-1264), a member of the EGF receptor (type 1) family involved in cell growth regulation. Expression or function may be altered in human cancers.

免疫原

Synthetic peptide sequence RSTLQHPDYLQEYST from the cytoplasmic domain of the protein, residues 1250-1264.
Epitope: Cytoplasmic domain of the protein, residues 1250-1264.

应用

Detect erbB4 using this Anti-erbB4 Antibody, clone HFR1/2G4 validated for use in WB, IP, IH(P).
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: Effective for formalin-fixed, paraffin-embedded sections.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of erbB-4/HER-4 Monoclonal Antibody: DCIS and Pancreatic Cancer
Research Sub Category
Growth Factors & Receptors
Research Category
Signaling

Apoptosis & Cancer

质量

Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected C-ERB-4 on 10 μg of A431 lysates.

目标描述

~147 kDa

联系

Replaces: 04-348

外形

Purified mouse monoclonal IgM in 0.02 M PB with 0.25 M NaCl, pH 7.6, 0.01% Sodium Azide.
Purified
Format: Purified

储存及稳定性

Stable refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

分析说明

Control
A431 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	HFR1/2G4, monoclonal
species reactivity	human, rat
species reactivity (predicted by homology)	mouse
technique(s)	immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1, kappa
NCBI登记号	NP_005226.1
UniProt登记号	Q15303
运输	wet ice
Gene Information	human ... ERBB4(2066)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable