半岛bd体育手机客户端 说明
一般描述
Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3′-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3′-UTR.
特异性
CUG-BP1
免疫原
Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50
应用
Anti-CUGBP1 Antibody, clone 3B1 is a high quality Mouse Monoclonal Antibody for the detection of CUGBP1 & has been validated in EMSA, IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
RNA Binding Protein (RBP)
质量
routinely evaluated by immunoblot on HeLa nuclear extract
目标描述
50kDa
外形
Protein G Chromatography
Format: Purified
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
储存及稳定性
2 years at -20°C
分析说明
Control
HeLa lysates
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 3B1, monoclonal |
species reactivity | rat, rabbit, human, bovine, mouse, pig |
manufacturer/tradename | Upstate? |
technique(s) | electrophoretic mobility shift assay: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_006560 (and others) |
UniProt登记号 | Q92879 |
运输 | wet ice |
Gene Information | human ... CELF1(10658) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich