半岛bd体育手机客户端 说明
一般描述
CFTR (Cystic Fibrosis Transmembrane conductance Regulator) is a 170 kDa chloride (Cl-) channel that regulates salt and water transport across epithelial tissues, where it modulates production of mucus, sweat, saliva, tears, and digestive enzymes. Cystic fibrosis patients have mutations in this protein, resulting in defective in ion transport. Symptoms include reduced transport of sodium chloride (saltier sweat) and digestive and respiratory disorders caused by thicker mucus. CFTR mutations are also associated with congenital bilateral absence of the vas deferens in males.
特异性
Other species cross-reactivity not tested.
This antibody recognizes CFTR, Mr 170kDa. Two additional proteins were detected, Mr 52 and 38 kDa, in CFTR-transfected BHK cell lysates.
免疫原
Synthetic peptide (RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). The immunizing sequence is identical in monkey and rabbit, has 11/12 identical amino acids in sheep, cow and frog, 10/12 identity in mouse and 9/12 identity in rat. Clone MM13-4.
应用
Immunoprecipitation:
This antibody has been shown to immunoprecipitate CFTR from a T84 cell lysate by an independent laboratory.
Immunocytochemistry:
This antibody has been reported to immunostain CFTR in T84 cells.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Anti-CFTR Antibody, clone MM13-4 detects level of CFTR & has been published & validated for use in IC, IP & WB.
质量
Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or CFTR-transfected BHK.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected CFTR in human T84 colon carcinoma epithelial RIPA cell lysates. A previous lot detected CFTR in CFTR-transfected BHK.
Note: CFTR can run as a diffuse protein on SDS-PAGE.
目标描述
170 kDa
外形
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1k in buffer containing 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Pancreas. RIPA cell lysates
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | MM13-4, monoclonal |
species reactivity | human |
manufacturer/tradename | Upstate? |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NM_000492 |
UniProt登记号 | P13569 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich