Thymidine Phosphorylase, recombinant fromEscherichia coli

半岛bd体育手机客户端 说明

一般描述

Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

应用

Thymidine phosphorylase has been used in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy. Thymidine phosphorylase has also been used in a study to investigate implications for the clinical efficacy of nucleoside analogues.

生化/生理作用

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. TheE. colithymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purifiedE. colienzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

单位定义

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25℃.

外形

Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin

制备说明

Cloned fromE. coliand produced in overexpressingE. coli

基本信息

酶学委员会(EC)编号	2.4.2.4 ( BRENDA | IUBMB )
MDL编号	MFCD00132426
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	300
重组	expressed inE. coli
无菌性	0.2 μm filtered
形式	buffered aqueous solution
浓度	≥900 units/mL
UniProt登记号	P07650
储存温度	2-8℃
Gene Information	Escherichia coliK12 ... deoA(948901)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)