半岛bd体育手机客户端 说明

一般描述

Interleukin-8 or CXCL8 (IL-8) is a proinflammatory chemokine that belongs to the C-X-C family. Monocytes and macrophages are the primary sources of IL-8 but it is also secreted by most nucleated cells. IL-8 binds to two G protein-coupled receptors, CXCR1 and CXCR2 and leads to the downstream activation of PI3K, MAPK, PKC, STAT3 and FAK pathways. IL-8 is responsible for the recruitment monocytes and neutrophils that results in a prolonged acute inflammation. The expression of IL-8 is influenced by inflammatory signals, chemical and environmental stress and hormones. Deregulation of IL-8 signaling has been observed in atherosclerosis, cardiovascular disease and solid tumors of bladder, prostate, ovary, glial cells and pancreas. Monoclonal Anti-Interleukin-8 antibody recognizes human IL-8. By immunoblotting, the antibody shows 100% cross reactivity with recombinant porcine IL-8, and no cross reactivity with recombinant rat CINC-2α.

免疫原

purified,E. coli-derived, recombinant human IL-8.

应用

Monoclonal Anti-Interleukin-8 antibody may be used for neutralization assays; ND50 is 0.08-0.4 μg/ml. The antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA a working concentration of 0.5 μg/ml may be used. Antibody concentration of 25 μg/ml may be used for immunocytochemistry using stimulated human peripheral blood mononuclear cells. For flow cytometry, 10 μL of 25 μg/ml stock antibody solution may be used for 1-5 X 10⁵cells.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00212220
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	6217, monoclonal
形式	lyophilized powder
species reactivity	human
technique(s)	capture ELISA: suitable flow cytometry: suitable immunocytochemistry: 25 μg/mL using using calcium ionophore- and PMA-stimulated human peripheral blood mononuclear cells neutralization: suitable western blot: 1-2 μg/mL
同位素/亚型	IgG1
储存温度	?20℃
Gene Information	human ... IL8(3576)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)