Thrombopoietin from mouse

半岛bd体育手机客户端 说明

应用

Thrombopoietin from mouse can be used in a genetic screening test for examining the role of somatic activating mutations in EPOR, MPL, or GCSFR genes in activating myeloproliferative disorders (MPD) in JAK2V617F-negative MPD patients.

生化/生理作用

Thrombopoeitin (TPO), also known as the ligand for thec-mplproto-oncogene (Mpl-L) and as megakaryocyte colony stimulating factor (Meg-CSF), is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. Produced mainly by hepatocytes but also by kidney cells, TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO) and prolactin (PRL). Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation. Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlpof the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp(TPO receptor). TPO receptors are found on megakaryocytes and their precursors and on platelets. The mature form of TPO is a highly conserved glycoprotein, showing approximately 70% sequence homology among various mammals. Human TPO contains 332 amino acids (mouse 335) including two internal disulfide bonds and 6 N-linked glycosylation sites (mouse 7). The N-terminal 153 amino acid region of TPO shows structural and amino acid sequence homology to erythropoietin and a pair of arginines (potential cleavage site) separating it from the highly glycosylated carboxyterminal domain.
Thrombopoietin from mouse is an amino acid peptide that plays a pivotal role in megakaryocyte proliferation and differentiation. It also serves as a growth factor for liver endothelial cells in mouse.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin

分析说明

The biological activity was measured in a cell proliferation assay using MO7e cells.

基本信息

MDL编号	MFCD01095336
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
重组	expressed in NSO cells
测定	>97% (SDS-PAGE)
形式	lyophilized powder
效能	2.1 ng/mL EC50
分子量	~75 kDa by SDS-PAGE (as a result of glycosylation) predicted mol wt 35 kDa
包装	pkg of 5 μg
储存条件	avoid repeated freeze/thaw cycles
technique(s)	cell culture | mammalian: suitable
杂质	endotoxin, tested
UniProt登记号	P40226
储存温度	?20℃
Gene Information	mouse ... Thpo(21832)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)