半岛bd体育手机客户端 说明
应用
Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 ℃ using a final concentration of 50 μM [32P] ATP.
生化/生理作用
Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.
单位定义
One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP
外形
Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
基本信息
| MDL编号 | MFCD07370971 |
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 重组 | expressed inE. coli |
| 测定 | ≥85% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| enzyme activity | ≥10 units/mg protein |
| 分子量 | protein apparent mol wt ~80 kDa |
| UniProt登记号 | P51955 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... NEK2(4751) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
