半岛bd体育手机客户端 说明
应用
Photo-activatable sterol probe for the bioorthogonal tagging and enrichment of sterol-binding biomolecules. This sterol probe contains an intact steroid core structure bearing a diazirine UV-activatable cross-linking group, as well as an alkyne group for click-chemistry mediated adduction of reporter and affinity tags. The probes are cell-permeable, allowing forin situlabeling of biomolecules in live cells, as well as in cell extractsin vitro.
包装
5 mg in glass bottle
其他说明
Handle the probes in amber vessels under dim ambient light whenever possible. Aqueous solutions of probes complexed to methyl-β-cyclodextrin (20:1 =m-β-CD:probe molar ratio, 5-20 μM probe) permeate cultured cells in complete serum-containing medium within 30 minutes at 37℃ and are cross-linked using near-UV (365 nm) light for 3-10 minutes. Cells can then be harvested and lysed, fractionated or otherwise processed. Click chemistry (Cu(I)-catalyzed 1,3-dipolar cycloaddition of the alkyne with an azide reporter tag such as a fluorophore or biotin, products760765or762024) can be performed in protein samples in PBS for gel-visualization of probe-labeling, MS-based proteomics analysis of probe-mediated affinity enrichments or western blot analysis.
基本信息
| 经验(实验)分子式 | C30H46N2O3 |
| 分子量 | 482.70 |
半岛bd体育手机客户端 性质
| 测定 | 95% |
| 质量水平 | 100 |
| 形式 | semisolid |
| reaction suitability | reaction type: click chemistry |
| 储存温度 | ?20℃ |
| SMILES string | C[C@H](CCC(=O)OCCCCC#C)[C@H]1CCC2C3CC4(N=N4)[C@@H]5C[C@H](O)CC[C@]5(C)C3CC[C@]12C |
| InChI | 1S/C30H46N2O3/c1-5-6-7-8-17-35-27(34)12-9-20(2)23-10-11-24-22-19-30(31-32-30)26-18-21(33)13-15-29(26,4)25(22)14-16-28(23,24)3/h1,20-26,33H,6-19H2,2-4H3/t20-,21-,22?,23-,24?,25?,26-,28-,29-/m1/s1 |
| InChI key | ZJMZIZZWLLQHJO-HFMSFSOYSA-N |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
