Granulocyte-Macrophage Colony-Stimulating Factor from rat

半岛bd体育手机客户端 说明

生化/生理作用

Four distinct colony-stimulating factors (CSFs) promoting survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and interleukin-3 (IL-3, Multi-CSF). GM-CSF is a multipotential growth factor, stimulating proliferation of progenitor cells from more than one hematopoietic lineage (granulocyte, macrophage, and eosinophil). GM-CSF stimulates colony formation from pluripotent progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. GM-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It is produced by various cell types (activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to cytokine and inflammatory stimuli. In addition to granulocyte-macrophage progenitors, GMCSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF can induce nonhematopoietic cells such as endothelial cells to migrate and proliferate. It also stimulates the proliferation of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity GM-CSF receptors contain a GM-CSF-specific α?chain and a common α ?chain.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

制备说明

Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced from a DNA sequence encoding mature rat GM-CSF. This recombinant protein is a mixture of two rat GM-CSFs, the 127 amino acid form and the 128 amino acid methionyl form. Mature rat GM-CSF has a calculated mass of 14.7 kDa. GM-CSF, an acidic glycoprotein, is species-specific.

分析说明

The biological activity is measured by its ability to induce proliferation of the factor-dependent mouse cell line, DA3.

基本信息

MDL编号	MFCD01868356
NACRES	NA.77

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
重组	expressed inE. coli
测定	>97% (SDS-PAGE)
形式	lyophilized powder
效能	0.03-0.1 ng/mL
质量	endotoxin tested
分子量	14.7 kDa
包装	pkg of 5X5 μg pkg of 5 μg
储存条件	avoid repeated freeze/thaw cycles
杂质	<1 EU/μg
UniProt登记号	P48750
储存温度	?20℃
Gene Information	rat ... CSF2(116630), Csf2(116630)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable