半岛bd体育手机客户端 说明
一般描述
A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨmin cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨminduced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨmin cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨminduced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
包装
5 mg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria
警告
Toxicity: Irritant (B)
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.
其他说明
Mathur, A., et al. 2000.Cardiovasc. Res.46,126.
Gravance, C.G., et al. 2000.Theriogenology53,1691.
Chanda, B., and Mathew, M.K. 1999.Biochim Biophys. Acta1416,92.
Dedov, V.N., and Roufogalis, B.D. 1999.FEBS Lett.456,171.
Polla, B.S., et al. 1996.Proc. Natl. Acad. Sci. USA93,6458.
White, R.J., and Reynolds, I.J. 1996.J. Neurosci.16,5688.
Ankacrona, M., et al. 1995.Neuron15,961.
Reers, M., et al. 1995.Methods Enzymol.260,406.
Cossarizza, A., et al. 1993.Biochem. Biophys. Res. Commun.197,40.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C25H27Cl4IN4 |
| 分子量 | 652.23 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze protect from light |
| 颜色 | red-orange |
| 溶解性 | DMSO: 5 mg/mL |
| 运输 | ambient |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
