半岛bd体育手机客户端 说明
一般描述
A potent and selective inhibitor of cyclin-dependent kinases (Cdks). Exhibits greater inhibitory potency compared to olomoucine (Cat. No. 495620). Inhibits p34 Cdc2/cyclin B (IC50= 650 nM), p33 Cdk2/cyclin A (IC50= 700 nM), p33 Cdk2/cyclin E (IC50= 700 nM), and p33 Cdk2/p35 (IC50= 200 nM) by competing for the ATP binding domain of these kinases. Exhibits very low sensitivity towards related kinases, such as Erk1 and Erk2 (IC50= 34 ?M and 14 ?M, respectively). Roscovitine does not significantly affect the activity of other protein kinases even at 100 ?M and compared to olomoucine, roscovitine displays increased anti-mitotic activity at the G1/S and G2/M phases of the cell cycle.
包装
5 mg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Reversible: yes
Primary Target
p34cdk1/cyclin B
Product competes with ATP.
Target IC50: 650 nM, 700 nM, 700 nM, and 200 nM against p34cdk1/cyclin B, p33cdk2/cyclin A, p33cdk2/cyclin E, and p33cdk5/p35, respectively
Cell permeable: no
警告
Toxicity: Irritant (B)
外形
A 50 mM (5 mg/282 ?l) solution of Roscovitine (Cat. No. 557360) in DMSO.
重悬
Following initial use, aliquot and freeze (-20°C). Once opened, the DMSO aliquots are stable for up to 6 months at -20°C.
其他说明
Meijer, L. 1996.Trends Cell Biol.6,393.
Rudolph, B., et al. 1996.EMBO J.15,3053.
法律信息
Sold under license of U.S. Patent 6,316,456.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C19H26N6O |
| 分子量 | 354.45 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | liquid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | 188.6 °F - (Dimethylsulfoxide) |
| 闪点(C) | 87 ℃ - (Dimethylsulfoxide) |
Sigma-Aldrich
m.cnreagent.com
