Elastase Substrate IV, Colorimetric-Calbiochem

半岛bd体育手机客户端 说明

一般描述

A specific substrate for pancreatic elastase (k_m= 100 ?M; k_cat/ K_m= 35,000 M^-1s^-1for rat pancreatic elastase, K_m= 30 ?M; k_cat/K_m= 35,000 M^-1s^-1for porcine). Cleavage of substrate can be monitored at ~405 nm.
A specific substrate for pancreatic elastase (K_m= 100 ?M; K_cat/K_m= 35,000 s^-1M^-1for rat pancreatic elastase; K_m= 30 ?M; K_cat/K_m= 351,000 s^-1M^-1for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol:see Largman, C. (1983)

包装

5 mg in Plastic ampoule

生化/生理作用

km = 100 ?M; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 ?M; kcat/Km = 35,000 M-1 s-1 for porcine
Product does not compete with ATP.
Primary Target
A specific substrate for pancreatic elastase
Reversible: no
Cell permeable: no

警告

Toxicity: Standard Handling (A)

序列

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

外形

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

重悬

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

其他说明

Largman, L. 1983.Biochemistry22,3763.
Del Mar, E.G., et al. 1980.Biochemistry19,468.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C25H34N6O9
分子量	562.57

半岛bd体育手机客户端 性质

质量水平	100
测定	≥95% (HPLC)
形式	lyophilized solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles desiccated
颜色	white to off-white
溶解性	ethanol: 1 mg/mL water: soluble
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable