半岛bd体育手机客户端 说明
一般描述
Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.
包装
5 mg in Plastic ampoule
生化/生理作用
Primary Target
Fluorogenic caspase-1 substrate
Product does not compete with ATP.
Reversible: no
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
Ac-Tyr-Val-Ala-Asp-AFC
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
Jones, R.A., et al. 1998.Hepatology27,1632.
Thornberry, N.A., and Lazebnik, Y. 1998.Science281,1312.
Walker, N.P., et al. 1994.Cell78,343.
Thornberry, N.A., et al. 1992.Nature356,768.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C33H36F3N5O10 |
| 分子量 | 719.66 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | 98% (HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze desiccated protect from light |
| 颜色 | white to off-white |
| 溶解性 | DMSO: 5 mg/mL |
| 荧光 | λex~400 nm λem~505 nm |
| 运输 | ambient |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
