半岛bd体育手机客户端 说明
一般描述
A cell-permeable purine compound that acts as a high affinity AhR antagonist (IC50= 127 nM against 3 nM TCDD-induced luciferase reporter activity in HepG2 cultures), but not a panel of 61 kinases. AhR expression and function knockouts by shRNAs, CH-223191 (Cat. No. 182705), or SR1 treatment all result in enhanced and sustained CD34+population in long-term proliferating/expanding cultures of cord blood-derived HSCs (hematopoietic setem cells) in serum-free media containing Tpo (Cat. No. 605218), SCF (Cat. No. 569600), Flt3 Ligand (Cat. No. 343010), and IL-6 (Cat. No. 407652) in a reversible manner. SR1 is reactive toward human, monkey, and canine, but not rat or murine, species.
A cell-permeable purine compound that acts as a high affinity (IC50= 40 nM against [125I]-N3Br2DD binding to human AhR) AhR antagonist (IC50= 127 nM against 3 nM TCDD-induced luciferase reporter activity in HepG2 cultures), while exhibiting little or no activity against a panel of 61 kinases. AhR expression and function knockouts by shRNAs, CH-223191 (Cat. No. 182705), or SR1 treatment all result in enhanced and sustained CD34+population in long-term proliferating/expanding cultures of cord blood-derived HSCs (hematopoietic setem cells) in serum-free media containing Tpo (Cat. No. 605218), SCF (Cat. No. 569600), Flt3 Ligand (Cat. No. 343010), and IL-6 (Cat. No. 407652) in a reversible manner (11- and 73-fold increase of total nucleated and CD34+cells, respectively, comparing 1 to 0 ?M SR1-treated cultures on day 21; 1118-fold increase in CD34+cells in 1 ?M SR1 cultures from day 0 to 21). SR1-expanded CD34+cells retain multilineage potential and the SR1-expanded progeny derived from 300 human CB CD34+cells are reported to exhibit the same level of engraftment efficiency as 10,000 unexpanded CB CD34+cells in NSG SCID micein vivo. SR1 is reactive toward human, monkey, and canine, but not rat or murine, species.
包装
5 mg in Glass bottle
Packaged under inert gas
警告
Toxicity: Regulatory Review (Z)
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Boitano, A.E., et al. 2010.Science329,1345.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C24H23N5OS |
分子量 | 429.54 |
MDL编号 | MFCD16987861 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
测定 | ≥98% (HPLC) |
形式 | solid |
manufacturer/tradename | Calbiochem? |
储存条件 | OK to freeze protect from light |
颜色 | white |
溶解性 | DMSO: 100 mg/mL |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich