PDK1, His·Tag^?, Human, Recombinant,S. frugiperda

半岛bd体育手机客户端 说明

一般描述

Full length, recombinant, human PDK1 fused to a His?Tag sequence and expressed inS. frugiperdainsect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKC_ζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.

包装

5 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.

外形

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 ?M EDTA, 500 ?M EGTA, 20% glycerol, 0.05% TRITON^?X-100 Detergent, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Scott, M.T., et al. 2002.EMBO J.21,6771.
Biondi, R.M., et al. 2000.EMBO J.19,979.
Alessi, D.R., et al. 1997.Curr. Biol.7,776.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

基本信息

酶学委员会(EC)编号

2.7.10

半岛bd体育手机客户端 性质

测定	≥90% (SDS-PAGE)
质量水平	100
形式	liquid
specific activity	≥75 units/mg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2