半岛bd体育手机客户端 说明
一般描述
A cell-permeable, reversible, and selective inhibitor of the phosphorylation of Akt and itsin vitrokinase activity (complete inhibition <5 M) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC50= 2-5 M), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent. Also shown to induce neuronal autophagy in an Akt- and mTOR-independent manner and enhances the clearance of misfolded protein. Also available as a 20 mM solution in H2O(Cat. No. 124039).
A cell-permeable, reversible, and selective inhibitor of the phosphorylation of Akt and itsin vitrokinase activity (complete inhibition <5 ?M) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC50= 2-5 μM), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent. Also shown to induce neuronal autophagy in an Akt- and mTOR-independent manner and enhances the clearance of misfolded protein.
包装
5 mg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Product does not compete with ATP.
Primary Target
Akt
Reversible: yes
Secondary Target
Rn cell lines (IC?? = 2-5 ?M)
Cell permeable: yes
Target IC50: <5 ?M against Akt ; 2-5 ?M against growth of Rh (rhabdomyosarcoma) cell lines
警告
Toxicity: Irritant (B)
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
Tsvetkov, A.S., et al. 2010.Proc. Natl. Acad. Sci. USAin press.
Thimmaiah, K.N., et al. 2005.J. Biol. Chem.280,31924.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C20H25ClN2O · xHCl |
| 分子量 | 344.88 (free base basis) |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
| 颜色 | white |
| 溶解性 | water: 1 mg/mL |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
