(±)-Blebbistatin-CAS 674289-55-5-Calbiochem

半岛bd体育手机客户端 说明

一般描述

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 ?M) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.
A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 ?M) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 ?l) solution of (±)-Blebbistatin (Cat. No. 203389) in 90% DMSO is also available.

包装

5 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Cell permeable: yes
Primary Target
ATPase
Product does not compete with ATP.
Target IC₅₀: <100 ?M inhibiting the ATPase and gliding motility of human platelets
Reversible: yes

重悬

Following reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

其他说明

Shu, S., et al. 2005.Proc. Natl. Acad. Sci. USA102,1472.
Kovacs, M., et al. 2004.J. Biol. Chem.279,35557.
Straight, A.F., et al. 2003.Science299,1743.
Cheung, A., et al. 2001.Mol. Biol. Cell Suppl.12,271a.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C18H16N2O2
分子量	292.33
NACRES	NA.77

半岛bd体育手机客户端 性质

质量水平	100
测定	≥97% (HPLC)
形式	solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze protect from light
颜色	yellow
溶解性	methanol: 1.5 mg/mL 100% DMSO: 100 mg/mL 90% DMSO: 75 mg/mL
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable