Late Endosome Trafficking Inhibitor, EGA-Calbiochem

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一般描述

A cell-permeable semicarbazone compound that preventsB. anthraxLethal toxin/LT (Protective Antigen/PA & LF/Lethal Factor complex; EC₅₀= 1.7 ?M in RAW264.7 cultures; EC_max= 6 ?M in murine BMDM cultures), diphtheria toxin (DT), LF-DT A chain fusion LFnDTA/PA complex,P. aeruginosaExoA, andH. ducreyicytolethal distending toxin (Hd-CDT) from exerting their cytotoxic activity, but not endocytic uptake, by blocking endosomal trafficking/maturation into acidified late endosomes, while exhibiting no cytoprotective efficacy against toxins that do not require acidified endosomes for intoxication, such as ricin &E. coliEc-CDT. Likewise, EGA is also shown to inhibit low-pH-dependent viral infectivity by Armstrong strain of LCMV, A/WSN/33 strain of influenza virus, vesicular stomatitis virus VSV-pseudotyped lentivirus & VSV-pseudotyped MLV, but not low-pH-independent infection of Nipah virus NiV or MLV. Although the exact molecular target remains unknown, EGA does not act as a pH neutralizing agent, nor does it inhibit endosomal recycling or trafficking to phagocytic compartments.
A cell-permeable semicabazone compound that preventsB. anthraxLethal toxin/LT (EC₅₀= 1.7 ?M in RAW264.7 cultures; EC_max= 6 ?M in murine BMDM cultures), diphtheria toxin/DT,P. aeruginosaExoA, andH. ducreyicytolethal distending toxin (Hd-CDT) from exerting their cytotoxic activity, but not endocytic uptake, by blocking endosomal trafficking/maturation into acidified late endosomes, while exhibiting no cytoprotective efficacy against ricin &E. coliEc-CDT that do not require acidified endosomes for intoxication. Likewise, EGA is also shown to inhibit low-pH-dependent viral infectivity by Armstrong strain of LCMV, A/WSN/33 strain of influenza virus, but not low-pH-independent infection of Nipah virus NiV or MLV. Although the exact molecular target remains unknown, EGA does not act as a pH neutralizing agent, nor does it inhibit endosomal recycling or trafficking to phagocytic compartments.

生化/生理作用

Cell permeable: yes
Primary Target
host-membrane trafficking
Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

重悬

Use only fresh DMSO for reconstitution.

其他说明

Gillespie, E.J., et al. 2013.Proc. Natl. Acad. Sci. USA110,E4904.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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