Diaphanous (mDia)-related Formin Agonist, IMM01

半岛bd体育手机客户端 说明

一般描述

A hydrazinecarbothioamide derived intramimic that acts as an agonist of mammalian Diaphanous (mDia)-related formins and disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC₅₀= 140 nM). Shown to trigger actin assembly and stabilize microtubules in a dose -dependent manner and at levels similar to those induced by Taxol. Induces caspase-3 activation and apoptosis in NIH 3T3 and SW480 cells(~ 100 ?M) and slows the growth of SW480 xenograft tumors in mice (~ 25 mg/kg). Shown to induce LacZ expression, producing b-gal activity that is comparable to effects seen with cytochalasin D treatment.
A hydrazinecarbothioamide derived intramimic that acts as an agonist of mammalian Diaphanous (mDia)-related formins and disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC₅₀= 140 nM). Shown to trigger actin assembly and stabilize microtubules in a dose -dependent manner and at levels similar to those induced by Taxol. Induces caspase-3 activation and apoptosis in NIH 3T3 and SW480 cells(~ 100 ?M) and slows the growth of SW480 xenograft tumors in mice (~ 25 mg/kg). Shown to induce LacZ expression, producing b-gal activity that is comparable to effects seen with cytochalasin D treatment.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

生化/生理作用

Primary Target
mDia
Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Lash, L.L., et al. 2013.Cancer Res.73,6793.
Copeland, S.J. et al. 2007.J Biol. Chem.282,30120.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C12H17N3O2S
分子量	267.35

半岛bd体育手机客户端 性质

质量水平	100
测定	≥98% (HPLC)
形式	powder
效能	140 nM IC50
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze protect from light
颜色	off-white
溶解性	DMSO: 100 mg/mL
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable