SKP2 E3 Ligase Inhibitor I, C1-CAS 432001-69-9-Calbiochem

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一般描述

A cell-permeable rhodanine compound that upregulates cellular p21^Cip1/Waf1and p27^Kip1protein levels (10 ?M for 16 h in 501 Mel cultures) by preventing Skp2-Cks1-mediated substrate recruitment without affecting the F-box E3 ubiquitin ligase Skp2 interaction with Cks1 or the SCF (Skp1-cullin1-F-box) complex. Shown to inhibit the proliferation of human breast cancer MCF-7 and melanoma 501 Mel cells (IC₅₀= 8 and 30 ?M, respectively; 16 h incubation). Unlike SMIP004 (Cat. No. 500517), C1 does not affect cellular Cyclin E/CDK2 kinase activity or Skp2 level.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
A cell-permeable rhodanine compound that upregulates cellular p21^Cip1/Waf1and p27^Kip1protein levels (10 ?M for 16 h in 501 Mel cultures) by preventing Skp2-Cks1 (Cdc kinase subunit 1)-mediated substrate recruitment without affecting the F-box E3 ubiquitin ligase Skp2 interaction with Cks1 or the SCF (Skp1-cullin1-F-box) complex. Shown to inhibit the proliferation of human breast cancer MCF-7 and melanoma 501 Mel cells (IC₅₀= 8 and 30 ?M; 16 h incubation). Unlike SMIP004 (Cat. No. 567314), C1 does not affect cellular Cyclin E/CDK2 kinase activity or Skp2 level.

生化/生理作用

Cell permeable: yes
Primary Target
disrupts p27-Skp2 interaction
Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

重悬

Use only fresh DMSO for reconstitution.
Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.

其他说明

Rico-Bautista, E., and Wolf, D.A. 2012.Chem. Biol.19,1497.
Wu, L., et al. 2012.Chem. Biol.19,1515.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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