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mTOR Activator, MHY1485-Calbiochem

品牌
Sigma-Aldrich
货号
5.00554
规格纯度
mTOR Activator, MHY1485, CAS Number: 326914-06-1, is a cell-permeable activator of mTOR that inhibits autophagy by suppressing the fusion between autophagosomes and lysosomes.
价格
2357.38 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
mTOR Activator, MHY1485-Calbiochem
4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

A cell-permeable triazine derivative that is shown to increase cellular mTOR Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation level in rat liver Ac2F cells (1 to 2 ?M for 1 h), affecting culture viability only at much higher concentrations (by 20% after 24 h 20 ?M treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes. The mechanism of mTOR activation by MHY1485 is currently unknown.
A cell-permeable triazine derivative that is shown to increase cellular phosphorylation level of mTOR Ser2448 (20% and 42% above basal in 1 h, respectively, with 1 and 2 ?M MHY1485) and downstream substrate 4E-BP Thr37/46 (29% and 34% above basal in 1 h, respectively, with 1 and 2 ?M MHY1485) in rat liver Ac2F cells, affecting culture viability only at much higher concentrations (by 20% after 24 h 20 ?M treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F (117%, 150%, and 250% of vehicle control LC3-II/LC3-I ratio, respectively, with 0.5, 1, and 2 ?M treatment for 6 h; 142% and 172% of control ratio, respectively, with 2 ?M treatment for 1 h or 12 h) is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes as demonstrated by a reduction of starvation-induced AdGFP-LC3 and lysosome dye co-localization (27% and 32.7% above control co-localization in 6 h serum deprived cultures with or without 2 ?M MHY1485; 21% above control co-localization with 2 ?M MHY1485 treatment alone and without serum starvation). The mechanism of mTOR activation by MHY1485 is currently unknown.

生化/生理作用

Cell permeable: yes
Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Choi, Y. J., et al. 2012.PLoS. One.7,e43418.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式 C17H21N7O4
分子量 387.39

半岛bd体育手机客户端 性质

质量水平 100
测定 ≥97% (HPLC)
形式 solid
效能 ~2 μM IC50
manufacturer/tradename Calbiochem?
储存条件 OK to freeze
protect from light
颜色 pale yellow
溶解性 DMSO: 12.5 mg/mL
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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