半岛bd体育手机客户端 说明
一般描述
A cell-permeable, all-D-amino acid autophagy-inducing peptide based on the retro-inverso sequence of Tat-Beclin1 (Cat. No. 506048) that is expected to be more resistant to proteolytic degradation than Tat-Beclin1 and is shown to be more potent than Tat-Beclin1 in inducing autophagy in muscle, heart, and pancreas tissues in 6-wk-old GFP-LC3 transgenic mice (1.94-, 1.48-, and 1.16-fold of Tat-Beclin1 GFP-LC3 induction potency in respective tissue in 6 h; 20 mg/kg i.p.)in vivo. Only the retro-inverso peptide, but not Tat-Beclin1, is effective in reducing autophagy substrate p62 level in the brain tissue of 5-d-old neontal GFP-LC3 mice (20 mg/kg i.p.). The retro-inverso peptide treatment is also reported to greatly reduce brain viral titre in neonatal mice subjected to WNV (West Nile Virus) CNS (central nervous system) infection (>1000-fold reduction 6 d post infection; 20 mg/kg/d i.p. beginning 1 d post infection), while being ineffective against WNV CNS infection in mice with monoallelic deletion of the Beclin1 geneBecn1.
生化/生理作用
Cell permeable: yes
Primary Target
GAPR-1/GLIPR2
Reversible: yes
包装
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
序列
(D)-H-arg-arg-arg-gln-arg-arg-lys-lys-arg-Gly-tyr~Gly-Gly~thr-Gly-phe-glu-Gly-asp-his-trp-ile-glu-phe-thr-ala-asn-phe-val-asn-thr-OH
外形
Supplied as a trifluoroacetate salt.
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Shoji-Kawata, S., et al. 2013.Nature.494,201.
法律信息
Sold under license of US Patent 8,722,628.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C164H251N57O45 |
分子量 | 3741.10 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
测定 | ≥95% (HPLC) |
形式 | powder |
manufacturer/tradename | Calbiochem? |
储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
颜色 | white |
溶解性 | DMSO: 50 mg/mL water: 50 mg/mL |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich