BL21(DE3) Electrocompetent Cells

半岛bd体育手机客户端 说明

一般描述

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)

特点和优势

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 10⁹cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

组分

• BL21(DE3) electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for expression

基本信息

NACRES

NA.85

半岛bd体育手机客户端 性质

等级	for molecular biology
细胞转化	competent cell type: electrocompetent transformation efficiency: ≥5 × 109cfu/μg
运输	dry ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 3