半岛bd体育手机客户端 说明
一般描述
Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0.
This product is prepared from recombinant trypsin, porcine sequence and the lysine residues have been dimethylated to further restrict autolysis. It is naturally devoid of chymotryptic activity. This high quality trypsin is suitable for proteomics use.
Specific activity: >= 10,000 BAEE units per mg protein.
基本信息
| NACRES | NA.26 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 重组 | expressed inPichia pastoris |
| 测定 | ≥10,000 unit/mg |
| 形式 | lyophilized powder |
| 适用性 | suitable for mass spectrometry |
| UniProt登记号 | P00761 |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |     |
| 警示用语： | Danger |
| 危险声明 | H290 - H314 - H317 - H332 - H334 - H335 - H411 |
| 预防措施声明 | P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 补充剂危害 | EUH071 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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