半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 3N7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects E3 ubiquitin-protein ligase ZNF598. It targets an epitope from the internal region.
免疫原
His-tagged recombinant fragment corresponding to 250 amino acids from the internal region of human E3 ubiquitin-protein ligase ZNF598, isoform 1.
应用
Anti-ZNF598, clone 3N7 ZooMAb, Cat. No. ZRB1353, recombinant Rabbit monoclonal antibody that targets E3 ubiquitin-protein ligase ZNF598 and has been tested in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ZNF598 in human pancreas tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZNF598 in A431, HeLa, HEK293, and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
目标描述
E3 ubiquitin-protein ligase ZNF598 (UniProt: Q86UK7; also known as EC:2.3.2.27, Zinc finger protein 598) is encoded by the ZNF598 gene (Gene ID: 90850) in human. ZNF598 is a E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation. It is required for ribosomes to terminally stall during translation of poly(A) sequences by mediating monoubiquitination of 40S ribosomal protein RPS10/eS10, RPS20/uS10 and RPS3/uS3. It also acts as a component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation. ZNF598 has one RING type (aa 29-69) and a C2H2 type (aa 187-210) zinc finger domain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Garzia, A., et al. (2017). Nat. Commun. 8, 16056).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
生物来源 | rabbit (recombinant) |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 3N7, monoclonal recombinant monoclonal |
半岛bd体育手机客户端 线 | ZooMAb?learn more |
形式 | lyophilized |
分子量 | calculated mol wt 98.64 kDa observed mol wt ~110 kDa |
species reactivity | mouse, human |
包装 | pkg of 25 μL |
环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG |
环保替代半岛bd体育手机客户端 分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich