半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 8G9.2 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Vesicular Glutamate Transporter 2.
免疫原
Full-length recombinangt Rat Vesicular Glutamate Transporter 2 (VGLUT2).
应用
Anti-VGLUT2, clone 8G9.2 ZooMAb, Cat. No. ZMS1026, is a recombinant Mouse monoclonal antibody that targets Vesicular glutamate transporter 2 and is tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected VGLUT2 in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VGLUT2 in SH-SY5Y cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VGLUT2 in rat hippocampus and human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Vesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID: 84487) in rat. VGluT2 is a multi-pass membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons. Its N- and C-terminal tails (aa 1-71 and 499-582, respectively) are exposed at the cytoplasmic side. VGLUT1- and VGLUT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VGLUT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. Expression of VGLUT2 is reported to increase sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | mouse |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 8G9.2, monoclonal recombinant monoclonal |
半岛bd体育手机客户端 线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 64.58 kDa observed mol wt ~55 kDa |
species reactivity | mouse, human, rat |
包装 | antibody small pack of 25 μL |
环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG1 |
环保替代半岛bd体育手机客户端 分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
Sigma-Aldrich