半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2N10-H1 is a ZooMAb?rabbit recombinant monoclonal antibody that specifically detects human GDP-L-fucose synthase. It targets an epitope within 20 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human GDP-L-fucose synthase.
应用
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TSTA3 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected TSTA3 in MCF-7 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected TSTA3 in human testis and human kidney tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TSTA3 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound TSTA3 peptide with a KD of 1.7 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TSTA3, clone 2N10-H1 ZooMAb?, Cat. No. ZRB1198, is a recombinant Rabbit monoclonal antibody that specifically targets TSTA3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
目标描述
GDP-L-fucose synthase (UniProt: Q13630; also known as EC:1.1.1.271, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, Protein FX, Red cell NADP(H)-binding protein, Short-chain dehydrogenase/reductase family 4E member 1) is encoded by the GFUS (also known as SDR4E1, TSTA3) gene (Gene ID: 7264) in human. In mammals, fucosylated glycans play important roles in ABO blood group antigens, host-microbe interactions, and several human diseases, including rheumatoid arthritis, pancreatic cancer, and hepatocellular carcinoma. GDP-L-fucose synthase is a homodimeric protein of the NAD(P)-dependent epimerase/dehydratase family that is responsible for the last step of the major metabolic pathway resulting in GDP-L-fucose synthesis from GDP-D-mannose. It catalyzes a combined epimerase and NADPH-dependent reductase reaction, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. This reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4. GDP-L-fucose synthase is also reported to act as the main autoantigen for a brain-infiltrating T cell clone. It is recognized by cerebrospinal fluid-infiltrating CD4+ T cells from HLA-DRB3*-positive subjects. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Santos, R., et al. (2018). Sci. Trans. Med. 10(462); eaat4301; Zhou, H., et al. (2013). Acta Biochim. Biophys. Sinica. 45(9); 720-725).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 2N10-H1, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt ~ 35.89 kDa observed mol wt ~ 38 kDa |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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