Anti-TMPRSS2 Antibody, clone 1B20 ZooMAb^?Rabbit Monoclonal

半岛bd体育手机客户端 说明

一般描述

ZooMAb^?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^?antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1B20 is a Rabbit recombinant monoclonal antibody that specifically detects Transmembrane protease serine 2 (TMPRSS2). It targets an epitope within 18 amino acids from the C-terminal half, extracellular domain.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, extracellular domain of human Transmembrane protease serine 2 (TMPRSS2).

应用

Quality Control Testing
Evaluated by Western Blotting in DU145 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TMPRSS2 in DU145 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected TMPRSS2 in PC3 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound TMPRSS2 with a KD of 3.8 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TMPRSS2 in human prostate tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TMPRSS2 in MCF-7 and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Transmembrane protease serine 2 (UniProt: O15393; also known as Serine protease 10, TMPRSS2) is encoded by the TMPRSS2 (also known as PRSS10) gene (Gene ID: 7113) in human. TMPRSS2 is a single-pass type II membrane protein of the peptidase S1 family that contains a cytoplasmic domain (aa 1-84), a transmembrane domain (aa 85-105), and an extracellular domain (aa 106-492). Its peptidase S1 domain is localized to amino acids 256-489. It is reported to be proteolytically processed by an autocatalytic mechanism generating the transmembrane protease serine 2 non-catalytic chain (1-255) and the transmembrane protease serine 2 catalytic chain (256-492). TMPRSS2 is shown to proteolytically cleave and activate viral spike glycoproteins, which facilitate virus-cell membrane fusions. It is reported to facilitate human SARS coronavirus (SARS-CoV) infection via two independent mechanisms: proteolytic cleavage of ACE2 that promotes viral uptake and cleavage of coronavirus spike glycoprotein, which activates the glycoprotein for cathepsin L-independent host cell entry. TMPRSS2 is highly expressed in the prostate tissue and lower expression is observed in the epithelia of the colon, stomach, epididymis and breast tissue. Some expression has also been reported in pancreatic acini, hepatic bile ducts, testicular Leydig cells and the kidney. Its expression levels are significantly elevated in both neoplastic prostate and in the epithelium of prostatic hyperplasia. Two isoforms of TMPRSS2 have been described that are produced by alternative splicing. This ZooMAb^?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zang, R., et al. (2020). Sci. Immunol. 5(47); eabc3582; Heurich, A., et al. (2014). J. Virol. 88(2); 1293-1307).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	rabbit
重组	expressed in HEK 293 cells
偶联物	unconjugated
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	1B20, recombinant monoclonal
描述	recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线	ZooMAb?learn more
形式	lyophilized
分子量	calculated mol wt 53.86 kDa observed mol wt ~54 kDa
纯化方式	using Protein A
species reactivity	mouse, human
species reactivity (predicted by homology)	feline, monkey, bovine, canine, rat
包装	antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry.
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
technique(s)	affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable
同位素/亚型	IgG
表位序列	C-terminal half
Protein ID登记号	NP_005647
UniProt登记号	O15393
环保替代半岛bd体育手机客户端 分类	Aligned
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable