半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 19M23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase Tec. It targets an epitope within 19 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human Tyrosine protein kinase Tec.
应用
Affinity Binding Assay: A representative lot of this antibody bound Tec peptide with a KD of 1.2 x 10-5 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Tec in K562 cells.
ELISA Analysis: A representative lot specifically detected Tec peptide in ELISA application, but not peptides from five other proteins.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Tec, clone 19M23 ZooMAb, Cat. No. ZRB1344, is a recombinant Rabbit monoclonal antibody that specifically targets human Tyrosine Protein kinase Tec and has been tested for use in Affinity Binding Assay , ELISA, Immunocytochemistry, and Western Blotting.
目标描述
Tyrosine-protein kinase Tec (UniProt: P42680; also known as EC:2.7.10.2, Tec) is encoded by the TEC (also known as PSCTK4) gene (Gene ID: 7006) in human. The Tec family of protein kinases consists of five members, Tec, Btk, Itk/Emt/Tsk, Bmx and Txk/Rlk. They are abundantly expressed in hematopoietic cells and participate in the growth and differentiation of blood cells. Tec is a peripheral membrane protein that serves as a non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. It is activated by tyrosine phosphorylation by a wide range of cytokine stimulations. It regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells and is required for TCR-dependent IL2 gene induction. Following B-cell or T-cell receptors activation by antigen, it is shown to translocate to the plasma membrane through its PH domain (aa 4-111). Tec is reported to serve as a central regulator of the noncanonical caspase-8 inflammasome that is involve in the assembly and activation of the caspase-8 inflammasome in response to fungal pathogens but is not involved in anti-bacterial immune responses. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mano, H., et al. (1999). Cytokines Growth Factor Rev. 10(3-4); 267-280; Zwolanek, F., et al. (2014). PLoS Pathog. 10(12); e1004525.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit (recombinant) |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 19M23, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 73.58 kDa observed mol wt ~65 kDa |
| species reactivity | human |
| 包装 | pkg of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | ELISA: suitable affinity binding assay: suitable immunocytochemistry: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
