Anti-STX17 Antibody, clone 1B3 ZooMAb^?Rabbit Monoclonal

半岛bd体育手机客户端 说明

一般描述

ZooMAb^?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^?antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1B3 is a ZooMAb^?Rabbit recombinant monoclonal antibody that specifically detects Syntaxin 17 (STX17). It targets an epitope within 20 amino acids from the N-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal, cytoplasmic domain of human Syntaxin17 (STX17).

应用

Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STX17 in A431 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected STX17 in lysates from HepG2 cells, mouse testis tissue, and C2C12 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected STX17 in human kidney tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected STX17 in A431 and HeLa cells..
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Syntaxin-17 (UniProt: P56962; also known as STX17) is encoded by the STX17 gene (Gene ID: 55014) in human. STX17 is a multi-pass membrane protein with two cytoplasmic domains (aa 2-228 and 276-302), two helical domains (aa 229-249 and 255-275), and one lumenal domain (aa 250-254). STX17 is as an autophagosomal soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) that has a hairpin-type structure mediated by two transmembrane domains, each containing glycine zipper motifs. This structure contributes to its specific localization to completed autophagosomes. Mutations in the glycine residues on the glycine zipper-like motifs is shown to impair its localization to autophagosomes. Although STX17 is shown to localize to the endoplasmic reticulum and mitochondria under nutrient-rich conditions, it translocates to autophagosomes under condition of starvation. It is reported to interact with SNAP29 that is recruited to autophagosomes upon starvation, and VAMP8, the lysosomal SNARE. Depletion of either SNAP29 or VAMP8 is shown to block autophagic flux. STX17 also plays a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment and regulates transport between them. This ZooMAb^?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Viret, C., and Faure, M. (2019). Trends Cell Biol. 29(11); 1-3; Itakura, E., et al. (2012). Cell. 151(6); 1256-1269).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	rabbit
重组	expressed in HEK 293 cells
偶联物	unconjugated
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	1B3, recombinant monoclonal
描述	recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线	ZooMAb?learn more
形式	lyophilized
分子量	calculated mol wt 33.4 kDa observed mol wt ~38 kDa
纯化方式	using Protein A
species reactivity	human, mouse
species reactivity (predicted by homology)	monkey, bovine
包装	antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry.
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
technique(s)	immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable
同位素/亚型	IgG
表位序列	N-terminal cytoplasmic domain
Protein ID登记号	NP_060389
UniProt登记号	P56962
环保替代半岛bd体育手机客户端 分类	Aligned
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable