Anti-Spectrin α Antibody, clone 2G17 ZooMAb^?Rabbit Monoclonal

半岛bd体育手机客户端 说明

一般描述

ZooMAb^?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^?antibodies are reliably available and ready to ship when you need them.

特异性

Clone 2G17 is a Rabbit recombinant monoclonal antibody that specifically detects Spectrin a, non-erythroid. It targets an epitope within 24 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Spectrin a, non-erythroid.

应用

Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Spectrin in SH-SY5Y cell lysate.

Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Spectrin in HEK293 cells and in Rat Brain, Mouse Brain, and Human Brain tissue lysates.
Affinity Binding Assay: A representative lot of this antibody bound Spectrin with a KD of 6.7 x 10-7 in an affinity binding assay.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Spectrin in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Spectrin in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Spectrin alpha chain, non-erythrocytic 1 (UniProt: Q13813; also known as Alpha-II spectrin, Fodrin alpha chain, Spectrin non-erythroid alpha subunit) is encoded by the SPTAN1 (also known as NEAS, SPTA2) gene (Gene ID: 6709) in human. Non-erythroid Spectrin plays a role in stabilizing membrane structures, maintaining cell shape, and linking the cytoskeleton to plasma membranes or intracellular vesicles. Spectrin is a structural protein that is abundantly present in neurons of the central nervous system and is cleaved into signature fragments by proteases involved in necrotic and apoptotic cell death. It is one of the primary targets cleaved by activated caspases during apoptosis. The full-length protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa. Spectrin a is involved in secretion and it interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. It has two calcium-binding regions (aa 2336-2347 and 2379-2390). Its μ-calpain cleavage site is localized at Tyr-Gly (aa 1176-1177). Phosphorylation of Tyrosine 1176 is shown to reduce sensitivity to cleavage by calpain in vitro. Plasma levels of calpain-cleaved Spectrin a, N-terminal fragment (SNTF) serves as a useful marker for mild to severe traumatic brain injury and SNTF immunostaining can also reveal previously undetected traumatic brain injury. Mutations in SPTAN1 gene are known to cause early infantile epileptic encephalopathy that is characterized by seizures associated with hypsarrhythmia, profound mental retardation with lack of visual attention and speech development. This ZooMAb^?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Siman, R., et al. (2013). Front. Neurol. 4; Article 190; Johnson VE., et al (2016). Acta Neuropathol. 131(1); 115 135).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	rabbit
重组	expressed in HEK 293 cells
偶联物	unconjugated
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	2G17, recombinant monoclonal
描述	recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线	ZooMAb?learn more
形式	lyophilized
分子量	calculated mol wt 284.54 kDa observed mol wt ~260 kDa
纯化方式	using Protein A
species reactivity	human, rat, mouse
包装	antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry.
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
technique(s)	affinity binding assay: suitable immunofluorescence: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable
同位素/亚型	IgG
表位序列	C-terminus
Protein ID登记号	NP_003118
UniProt登记号	Q13813
环保替代半岛bd体育手机客户端 分类	Aligned
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable