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位置: 首页> 品牌> Sigma-Aldrich> Anti-Reelin Antibody, clone G10 ZooMAb?Mouse Monoclonal

Anti-Reelin Antibody, clone G10 ZooMAb?Mouse Monoclonal

品牌
Sigma-Aldrich
货号
ZMS1025
规格纯度
recombinant, expressed in HEK 293 cells
价格
2571.67 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Reelin Antibody, clone G10 ZooMAb ?Mouse Monoclonal
RELN
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone G10 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Reelin. It targets an epitope within the N-terminal region.

免疫原

A recombinant fragment corresponding to 333 amino acids from the N-terminal region of human Reelin.

应用

Anti-Reelin, clone G10 ZooMAb, Cat. No. ZMS1025, is a recombinant Mouse monoclonal antibody that specifically targets Reelin and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin)\, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Reelin in lysates from mouse E13-14 brain and rat E16 brain tissue.
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Reelin in C6 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Reelin in E16.5 embryonic mouse brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Reelin (UniProt: P78509; also known as RELN) is encoded by the RELN gene (Gene ID: 5649) in human. Reelin is a large extracellular matrix glycoprotein with serine protease activity. It plays a role in brain development and layering of neurons in the cerebral cortex and cerebellum. It is abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. It is also expressed in the retina, liver, thyroid gland, adrenal gland, fallopian tube, and breast tissue. Reelin is shown to regulate the positioning of neurons, as well as neurotransmission and memory formation. It also regulates microtubule function in neurons and neuronal migration and affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. It is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off. The mature form ahs 8 EGF-like domains and its Reelin domain is located in amino acids 26-190. Reelin participates in canonical signaling via binding to VLDL receptor and apolipoprotein E receptor 2 and induces tyrosine phosphorylation of the adaptor protein disabled 1 (Dab1) by Src kinases. Phosphorylated Dab1 in-turn activates downstream signaling, including the PI3-kinase-dependent pathways. Mutations in RELN gene have been linked to familial temporal lobe epilepsy and Lissencephaly that is associated with ataxia, mental retardation, and seizures. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mikulska-Ruminska, K., et al. (2019). Sci. Rep. 9; 18974; Dlugosz, P. and Nimpf, J. (2018). J. Mol. Sci. 19(10); E3090 ).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse (recombinant)
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 G10, monoclonal
recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 388.39 kDa
observed mol wt ~380-400 kDa
species reactivity mouse, rat
species reactivity (predicted by homology) human
包装 antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG1
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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