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位置: 首页> 品牌> Sigma-Aldrich> Anti-RAR-alpha Antibody, clone 3L14, ZooMAb?Rabbit Monoclonal

Anti-RAR-alpha Antibody, clone 3L14, ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1369
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-RAR-alpha Antibody, clone 3L14, ZooMAb ?Rabbit Monoclonal
Nuclear receptor subfamily 1 group B member 1
Retinoic acid receptor alpha
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3L14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Retinoic Acid Receptor alpha (RAR-alpha). It targets an epitope within 17 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Retinoic Acid Receptor alpha.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RAR-alpha in A431 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RAR-alpha in HeLa, A431, HUVEC, and NTERA2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected RAR-alpha in human prostate cancer and human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Retinoic Acid Receptor alpha peptide with a KD of 9.8 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-RAR-alpha, clone 3L14 ZooMAb, Cat. No. ZRB1369, a Rabbit monoclonal antibody that targets RAR-alpha and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

目标描述

Retinoic acid receptor alpha (UniProt: P10276; also known as RAR-alpha, Nuclear receptor subfamily 1 group B member 1) is encoded by the RARA (also known as NR1B1) gene (Gene ID: 5914) in human. RAR-alpha is a nuclear receptor of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. RAR-alpha forms heterodimer with RXR (via C-terminus) and this association is enhanced by pulsatile shear stress. The RAR-alpha/RXR heterodimer bind to the retinoic acid response elements composed of tandem 5′-AGGTCA-3′ sites known as DR1-DR5. Its nuclear localization depends on ligand binding, phosphorylation, and sumoylation. Phosphorylation on serine 77 is shown to be crucial for its transcriptional activity. Phosphorylation by Akt1 is required for the repressor activity but it has no effect on DNA binding, protein stability, or subcellular localization. In the absence of ligand, the heterodimer associates with a multiprotein complex containing transcription corepressors that induces histone deacetylation, chromatin condensation and transcriptional suppression. Upon ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RAR-alpha has two NR C-4 type of zinc-finger domains (aa 88-108 and 124-148). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit (recombinant)
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 3L14, monoclonal
recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 50.77 kDa
observed mol wt ~58 kDa
species reactivity human, canine
包装 pkg of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型 IgG
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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