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位置: 首页> 品牌> Sigma-Aldrich> Anti-phospho-ULK1-Ser757 Antibody, clone 5L18 ZooMAb?Rabbit Monoclonal

Anti-phospho-ULK1-Ser757 Antibody, clone 5L18 ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1621
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-phospho-ULK1-Ser757 Antibody, clone 5L18 ZooMAb ?Rabbit Monoclonal
Autophagy-related protein 1 homolog
hATG1
EC:2.7.11.1
Unc-51-like kinase 1
ATG1
Serine/threonine-protein kinase ULK1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 5L18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects ULK1 phosphorylated on serine 757.

免疫原

KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 757 from the C-terminal half of human ULK1.

应用

Peptide Inhibition Assay: Target band detection in lysate from serum starved A431 cells was prevented by preblocking of a representative lot (1:1,000 dilution) with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Affinity Binding Assay: A representative lot of this antibody bound phosphorylated ULK1 (pSer757) peptide with a KD of 8.2 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-phospho-ULK1-Ser757, clone 5L18 ZooMAb, Cat. No. ZRB1621, is a recombinant Rabbit monoclonal antibody that targets ULK1 phosphorylated on serine 757 and is tested in Affinity Binding Assay and Peptide Inhibition Assay, and Western Blotting.

目标描述

Serine/threonine-protein kinase ULK1 (UniProt: O75385; also known as EC:2.7.11.1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1) is encoded by the ULK1 (also known as KIAA0722) gene (Gene ID: 8408) in human. ULK1 is a ubiquitously expressed serine-threonine protein kinase that is involved in autophagy in response to starvation. During starvation it is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. In cells, ULK1 is constitutively in complex with at least three proteins: ATG13, FIP200 and ATG101. Interaction of ULK1 with ATG13 or FIP200 is reported to increase ULK1 kinase activity and stability. ULK1 undergoes autophosphorylation under nutrient-rich conditions and is dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, it is phosphorylated by MTOR/mTOR, which disrupts the interaction with AMPK and prevents activation of ULK1. In response to nutrient limitation it is phosphorylated and activated by AMPK that leads to activation of autophagy. It can also act as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulation of AMPK activity. Under nutrient sufficiency, high mTOR activity is reported to prevent ULK1 activation by phosphorylating ULK1 on serine 757 and disrupt its interaction with AMPK. The protein kinase domain of ULK1 is localized to amino acids 16-278. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zachari, M., and Ganley, IG. (2017). Essays Biochem. 61(6); 585-596; Kim, J., et al. (2011). Nat. Cell Biol. 13(2); 132-141).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 5L18, monoclonal
recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 112.63 kDa
observed mol wt ~112 kDa
species reactivity human
species reactivity (predicted by homology) bovine, sheep
包装 antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) affinity binding assay: suitable
inhibition assay: suitable (peptide)
western blot: suitable
同位素/亚型 IgG
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1

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