Anti-Nestin Antibody, clone 8B8, ZooMAb^?Rabbit Monoclonal

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 8B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Nestin. It targets an epitope with in 150 amino acids from the C-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal region of human Nestin.

应用

Anti-Nestin, clone 8B8, ZooMAb is a recombinant Rabbit Monoclonal antibody that detects Nestin and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nestin in SH-SY5Y cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nestin in lysate from Human H9 Stem Cells.

目标描述

Nestin (UniProt: P48681) is encoded by the NES (also known as Nbla00170) gene (Gene ID: 10763) in human. Nestin is a class VI intermediate filament protein that is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. Nestin expression is up-regulated in most mitotic cells and is downregulated upon differentiation. Upon terminal neural differentiation Nestin levels are shown to be down-regulated and replaced by neurofilaments. Nestin is also shown to be expressed in kidney in a developmentally regulated pattern. Its expression is also associated with tubulointerstitial injury and can serve as a marker for peritubular endothelial cell injury and tubulointerstitial fibrosis. Nestin is reported to be constitutively phosphorylated and its phosphorylation state increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Nestin has a short N-terminus and an unusually long C-terminus region that results in complex binding to an array of structural proteins. The short N-terminus leads to preferential intermediate filaments formation with heterodimers and heterotetramers and the long C-terminal portion functions as links or bridges between intermediate filaments and microtubules. Nestin can exist in homodimeric or homotetrameric form, however in mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, it can form heterodimers, which can assemble to form intermediate filaments. Nestin is shown to promote the disassembly of phosphorylated vimentin intermediate filaments during mitosis and play a role in the trafficking and distribution of intermediate filament proteins to daughter cells during progenitor cell division. (Ref.: Xie, L., et al. (2015). Stem Cells Int. 2015:762098; Tomioka, M., et al. (2010). 15(5); 568-574).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源	rabbit (recombinant)
重组	expressed in HEK 293 cells
偶联物	unconjugated
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	8B8, monoclonal recombinant monoclonal
半岛bd体育手机客户端 线	ZooMAb?learn more
形式	lyophilized
分子量	calculated mol wt 177.44 kDa observed mol wt ~ _220 kDa
species reactivity	human
包装	antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry.
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
technique(s)	immunocytochemistry: suitable using 1:100 immunohistochemistry: suitable using 1:100 western blot: suitable using 1:1,000
同位素/亚型	IgG
UniProt登记号	P48681
环保替代半岛bd体育手机客户端 分类	Aligned
运输	ambient
储存温度	2-8℃
Gene Information	human ... NES(10763)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1