半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2M18 is a ZooMAb?Rabbit recombinant monoclonal antibody that specifically detects Na+-/K+ ATPase a1. It targets an epitope within 16 amino acids from the N-terminal, first cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal, first cytoplasmic domain of human Na(+)/K(+) ATPase a1 subunit.
应用
Quality Control Testing
Evaluated by Western Blotting in Cos-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Na+/K+ ATPase 1 in Cos-7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Na+/K+ ATPase 1 in lysates from mouse testis tissue.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Na+/K+ ATPase 1 in one million HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Na+/K+ ATPase 1 in human colon tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Na+/K+ ATPase 1 in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Sodium/potassium-transporting ATPase subunit alpha-1 (UniProt: P05023; also known as EC:7.2.2.13, Na (+)/K (+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1) is encoded by the ATP1A1 gene (Gene ID: 476) in human. Na (+)/K (+) ATPase a1 is a multi-pass membrane protein with five extracellular domains, ten transmembrane domains, and six cytoplasmic domains. It is synthesized with a short propeptide (aa 1-5), which is removed in the mature form. It serves as the catalytic component of the active enzyme that catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions that provides energy for active transport of various nutrients. This gradient is also essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. Phosphorylation of Na (+)/K (+) ATPase a1 on tyrosine 10 modulates its pumping activity and phosphorylation on serine 943 by protein kinase A modulates its response protein kinase C. Following increase in intracellular sodium, it undergoes dephosphorylation by protein phosphatase 2A (PP2A) that leads to increase in its catalytic activity. Mutations in ATP1A1 gene have been linked to Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | rabbit |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 2M18, recombinant monoclonal |
描述 | recombinant, expressed in HEK 293 cells |
半岛bd体育手机客户端 线 | ZooMAb?learn more |
形式 | lyophilized |
分子量 | calculated mol wt 112.90 kDa observed mol wt ~100 kDa |
纯化方式 | using Protein A |
species reactivity | human, mouse, monkey |
species reactivity (predicted by homology) | bovine |
包装 | antibody small pack of 25 μL |
环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG |
表位序列 | N-terminal |
Protein ID登记号 | NP_000692 |
UniProt登记号 | P05023 |
环保替代半岛bd体育手机客户端 分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich