半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 3H17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects N-Cadherin. It targets an epitope within 18 amino acids from the C-terminal half, extracellular domain.
免疫原
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half, extracellular domain of human N-Cadherin.
应用
Anti-N-Cadherin, clone 3H17 ZooMAb, Cat. No. ZRB1126, is a recombinant Rabbit monoclonal antibody that targets N-Cadherin and has been tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected N-Cadherin in C2C12 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected N-Cadherin in C6 and SH-SY5Y cell lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected N-Cadherin in human liver tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Cadherin in PC12 and SH-SY5Y cells.Affinity Binding Assay: A representative lot of this antibody bound N-cadherin peptide with a KD of 8.5 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Cadherin-2 (UniProt: P19022; also known as CDw325, Neural cadherin, N-cadherin, CD325) is encoded by the CDH2 (also known as CDNH, NCAD) gene (Gene ID: 1000) in human. N-Cadherin is a single-pass type I membrane glycoprotein protein that plays a role in calcium-dependent cell adhesion. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. N-cadherin are synthesized as precursor proteins with a signal peptide (aa 1-265) and a propeptide (aa -26-159) that are proteolytically cleaved to generate functional, mature protein. Newly synthesized proN-cadherin (aa 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. The mature N-Cadherin contains an extracellular domain (aa 160-724) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (aa 725-745), and a conserved cytoplasmic domain (aa 746-906) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| 克隆 | recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 99.81 kDa observed mol wt ~ 130 kDa |
| species reactivity | rat, human, mouse |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
