您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-MMP14 Antibody, clone LEM-2/15.8 ZooMAb?Mouse Monoclonal

Anti-MMP14 Antibody, clone LEM-2/15.8 ZooMAb?Mouse Monoclonal

品牌
Sigma-Aldrich
货号
ZMS1060
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-MMP14 Antibody, clone LEM-2/15.8 ZooMAb ?Mouse Monoclonal
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone LEM-2/15.8 is a mouse recombinant monoclonal antibody that specifically detects Matrix metalloproteinase-14 (MMP14). It targets an epitope within 16 amino acids from the extracellular domain.

免疫原

Linear Peptide corresponding to 16 amino acids from the extracellular domain of human Matrix Metalloproteinase-14 (MMP14).

应用

Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MMP14 in HepG2 cell lysate.
Tested applications
Flow Cytometry Analysis: 1 μg from a representative lot detected MMP14 in one million HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MMP14 in human colon tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MMP14 in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MMP14, clone LEM-2/15.8 ZooMAb?, Cat. No. ZMS1060, is a recombinant Mouse monoclonal antibody that targets MMP14 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Matrix metalloproteinase-14 (UniProt: P50281; also known as EC:3.4.24.80, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP) is encoded by the MMP14 gene (Gene ID: 4323) in human. MMPs are a family zinc-dependent endoproteinases that degrade collagen and other structural proteins and are responsible for much of the turnover of matrix components. Each MMP contains a protease domain with a conserved HExGHxxGxxHS/T sequence, where three histidine residues form a complex with a catalytic Zinc atom. A regulatory domain with a conserved PRCGxPD motif helps to maintain MMPs in their latent form by binding of the cysteine residue to the active site Zn. MMP-14 is a single-pass type I membrane protein that is expressed in stromal cells of colon, breast, head, and neck. It is a membrane type matrix metalloproteinase (MT-MMP) that is synthesized with a signal peptide (aa 1-20) and a propeptide (aa 21-111) that are subsequently cleaved off to generate the active mature form. The propeptide maintains it in a latent state. The mature enzyme contains an extracellular domain (aa 112-541), a transmembrane domain (aa 542-562), and a cytoplasmic domain (aa 563-582). MMP14 contains four hemopexin domains within its extracellular domain and a cysteine switch (aa 91-98). The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the release of propeptide activates the enzyme. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 LEM-2/15.8, recombinant monoclonal
描述 recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 65.89 kDa
observed mol wt ~55 kDa
species reactivity human
species reactivity (predicted by homology) rat, bovine, monkey, mouse
包装 antibody small pack of 25 μL
增强验证 recombinant expression
Learn more aboutAntibody Enhanced Validation
technique(s) flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG1
表位序列 Extracellular domain
UniProt登记号 P50281
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map