半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1L17 is a Rabbit recombinant monoclonal antibody that specifically detects Histone Deacetylase 7 (HDAC7). It targets an epitope within 21 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Histone Deacetylase 7 (HDAC7).
应用
Quality Control Testing
Evaluated by Western Blotting in Rat Thymus tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected HDAC7 in Rat Thymus tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected HDAC7 in HeLa and NIH3T3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HDAC7 in human placenta tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HDAC7 in A431, HeLa, and NIH 3T3 cells.
Affinity Binding Assay: A representative lot of this antibody bound HDAC7 with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Histone deacetylase 7 (UniProt: Q8WUI4; also known as EC:3.5.1.98, HD7, Histone deacetylase 7A, HD7a) is encoded by the HDAC7 (also known as HDAC7A) gene (Gene ID: 51564) in human. Histone acetylation and deacetylation play a major role in determining chromatin structure and function. These modifications are achieved by histone acetyltransferases (HATs) and histone deacetylases (HDACs). In the deacetylated state, specific basic amino acids in histones are positively charged and interact with DNA s negatively charged phosphate groups and with negatively charged patches on neighboring nucleosomes. This promotes chromatin condensation to form heterochromatin. On the other hand, acetylation neutralizes these positive charges and promoting the less condensed and more accessible chromatin conformation known as euchromatin. HDAC7 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). It shuttles between the nucleus and the cytoplasm and in the nucleus, it associates with distinct subnuclear dot-like structures. Its nuclear export signal is localized in amino acids 918-952. Its deacetylase activity maps to a C-terminal domain and is dependent on its interaction with other class I HDACs in the nucleus. HDAC7 can undergo phosphorylation by PKN1 and PKN2 that impairs its nuclear import. Its phosphorylation on serine 155 by MARK2, MARK3, and PRKD1 is shown to promote its interaction with 14-3-3 proteins and facilitate its export from the nucleus. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | rabbit |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1L17, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 102.93 kDa observed mol wt ~110 kDa |
| 纯化方式 | using Protein A |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | monkey |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 表位序列 | N-terminal |
| Protein ID登记号 | NP_001091886 |
| UniProt登记号 | Q8WUI4 |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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