半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1D11 is a Rabbit recombinant monoclonal antibody that specifically detects Gasdermin-D. It targets an epitope within 25 amino acids from the internal region.
免疫原
KLH-conjugated linear peptide corresponding to 25 amino acids from the internal region of human Gasdermin-D.
应用
Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GSDMD in A431 cell lysate.
Tested applications
Affinity Binding Assay: A representative lot of this antibody bound GSDMD with a KD of 3.0 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GSDMD in human stomach tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GSDMD in A431, HeLa, HUVEC, and NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Gasdermin-D (UniProt: P57764; also known as Gasdermin domain-containing protein 1) is encoded by the GSDMD1 (also known as DFNA5L, GSDMDC1, FKSG10) gene (Gene ID: 79792) in human. Gasdermin-D is a member of the gasdermin family of proteins that promotes inflammatory cell death (pyroptosis) in response to microbial infection and danger signals. Its expression is observed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach. Gasdermin-2 consists of a 31 kDa N-terminal and a 22 kDa C-terminal domain that are separated by a linker region. Under normal conditions, the full-length Gasdermin-D is inactive as the linker loop between the N-terminal and C-terminal domains stabilizes the overall conformation of the full-length protein and allows the C-terminal domain to fold back on and auto-inhibit the N-terminal Gasdermin-D (Gasdermin-N). Upon interdomain cleavage by the action of caspase-1 and 4 at Asp275, the autoinhibition is relieved and Gasdermin-N cytotoxicity is triggered. Gasdermin-N is reported to form membrane pore that lead to cytokine release and pyroptosis. It also displays bactericidal activity. Gasdermin-N released from pyroptotic cells into the extracellular milieu can bind to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lieberman, J., et al. (2019). Sci. Immunol. 4(39); eaav1447; Liu, X., et al. (2016). Nature. 535(7610); 153-158).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | rabbit |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1D11, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 52.80 kDa observed mol wt ~50 kDa |
| 纯化方式 | using Protein A |
| species reactivity | mouse, human |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 表位序列 | Internal |
| Protein ID登记号 | NP_079012.3 |
| UniProt登记号 | P57764 |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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